Guess

[RoSa]

<center>

lets enjoy ..

tell us what can you see in this picture ..?
and if you have any few informations share them with us :)



So ..

GUESS what is this ??


http://css.sfu.ca/sites/nano/equipment/img/transmission-electron-microscope.jpg


Regards ..

[Asultan]

But I think it is optical microscope or transmission electron microscope

I think one of them... :85: :85:


I hope its correct ..

:eye:

[dr.ammooory]

TEMs are patterned after Transmission Light Microscopes and will yield similar information.

Morphology
The size, shape and arrangement of the particles which make up the specimen as well as their relationship to each other on the scale of atomic diameters.

Crystallographic Information
The arrangement of atoms in the specimen and their degree of order, detection of atomic-scale defects in areas a few nanometers in diameter
Compositional Information (if so equipped)
The elements and compounds the sample is composed of and their relative ratios, in areas a few nanometers in diameter

--------------------------------------------------------------------------------

A TEM works much like a slide projector. A projector shines a beam of light through (transmits) the slide, as the light passes through it is affected by the structures and objects on the slide. These effects result in only certain parts of the light beam being transmitted through certain parts of the slide. This transmitted beam is then projected onto the viewing screen, forming an enlarged image of the slide.
TEMs work the same way except that they shine a beam of electrons (like the light) through the specimen(like the slide). Whatever part is transmitted is projected onto a phosphor screen for the user to see. A more technical explanation of a typical TEMs workings is as follows (refer to the diagram below):

--------------------------------------------------------------------------------

The "Virtual Source" at the top represents the electron gun, producing a stream of monochromatic electrons.
This stream is focused to a small, thin, coherent beam by the use of condenser lenses 1 and 2. The first lens(usually controlled by the "spot size knob") largely determines the "spot size"; the general size range of the final spot that strikes the sample. The second lens(usually controlled by the "intensity or brightness knob" actually changes the size of the spot on the sample; changing it from a wide dispersed spot to a pinpoint beam.
The beam is restricted by the condenser aperture (usually user selectable), knocking out high angle electrons (those far from the optic axis, the dotted line down the center)
The beam strikes the specimen and parts of it are transmitted
This transmitted portion is focused by the objective lens into an image
Optional Objective and Selected Area metal apertures can restrict the beam; the Objective aperture enhancing contrast by blocking out high-angle diffracted electrons, the Selected Area aperture enabling the user to examine the periodic diffraction of electrons by ordered arrangements of atoms in the sample
The image is passed down the column through the intermediate and projector lenses, being enlarged all the way
The image strikes the phosphor image screen and light is generated, allowing the user to see the image. The darker areas of the image represent those areas of the sample that fewer electrons were transmitted through (they are thicker or denser). The lighter areas of the image represent those areas of the sample that more electrons were transmitted through (they are thinner or less dense)

[dr_perfecta]

gr8... I really like this kinds of light; useful threads....
I agree with the respected member above.... it is the TEM....

u took me several yrs back to the introdutory lec.in mico.labs.... & dr.Farooq ...dr.Mubasher.....

I remeber some of its application...especially how it differs from the light microscop.... because :O: it was an important question

OK...because the wavelength of electrons is much smaller than that of light, the optimal resolution attainable for TEM images is many orders of magnitude better than that from a light microscope. Thus, TEMs can reveal the finest details of internal structure - in some cases as small as individual atoms.

it can be used for biological and non biological specimens

For biological samples, cell structure and morphology is commonly determined whilst the localisation of antigens or other specific components within cells is readily undertaken using specialised preparative techniques.

For non-biological materials,
1-phase determination as well as defect and precipitate orientation are typical outcomes of conventional TEM experiments.
2-Microstructural characterisation of non-biological materials, including unit cell periodicities, can be readily determined using various combinations of imaging and electron diffraction techniques.

an what i really like about the TEM is that Images obtained from a TEM are two-dimensional sections of the material under study, but applications which require three-dimensional reconstructions can be accommodate by these techniques.


also if I 'm rememering right... it only found in high tech. & specialized centers....

thanx again.....

[RoSa]

<center>

Thank you all ( Asultan, dr-ammory & dr-perfecta ) for sharing us your nice information's...

Really I appreciate that...

Regards..

[RoSa]

<center>

WhAt Is ThIs ??

http://rosa.jeeran.com/3.jpg

[dr_perfecta]

Hi again.....

I think this a prepheral blood film showing....Target cells codocytes...which abnormal red blood cells

as the fegure u put Rosa....it shows red blood cells contain a bright white center encircled by a dark ring that makes it look like a target
The center of the cell does not pulsate or fade in due to the fact that the cell membrane has collapsed on itself , it remains static and bright resemble targets.
from where it took its name...

patogenesis
The presence of target cells or codocytes is associated with an altered surface membrane area to volume ratio.
-Absolute increases in surface membrane are usually attributed to disorders of lipid metabolism (decreased lecithin cholesterol acyltransferase activity, liver disease).
-Decreased cytoplasmic volume is associated with decreased production of hemoglobin (iron deficiency) or manufacture of defective hemoglobin (thalassemia).
-the appearance of target cells may occur when splenic function is absent (post-splenectomy) or impaired.


deffrenyial diagnosis of target cell in blood film...
1-Liver disease

2-Iron lack or deficiency

3-Post-splenectomy

4-Decreased lecithin cholesterol acyltransferase activity

5-Thalassemia (hemoglobinopathy)


dear collegue rosa....plz correct me if I'm wrong

thanx .....with regards

[RoSa]

<center>
dear dr.perfecta ..
sorry for lating in replay ..

you know the aim of this thread is to refresh and memorize our informations ..
and i think that i don't have the ability to correct any word writtin here ..
i need who support my informations :roll eyes:


i am really appreciating your begin with us ..
also dr.perficta u can add whatever u want

Regards ..

[RoSa]

<center>


WhAt Is ThIs ??


http://rosa.jeeran.com/Set1_4112.jpg

[Dr.6nash]

<center>

what is this???!!
from where u get this pic Rosa??
:o:

O.K its an embeding machine
that use to cover the fresh histology / cytology tissue with a paraffin wax
so that can easly cut it & being ready for processing..

u can see this machine in Pathology Lab M.S students
:sun:

[sweetone]

hi
how r u???all


it is very nice....method to learn.....to have experince also


thank u rosa


I wanna share with this picture if u dont mind rosa

[RoSa]

<center>
ahleeen dr.6anash ..

i was waiting you to put this pic. ,, but u didnt ..

so i put it by myself :p

thank you to be here :)


Regards ..

[RoSa]

<center>
sweetone
thank you to be here ...

ummmm ..

this pic. i don't have any idea about it :(

let me searching , asking , and waiting for the others ..


is it one of blood cells ?? or worms ?? :D ( ابووووك يالمعلوماااات )


Regards..

[sweetone]

hi all ?????


No one can guess this pic.

it is not difficult but not clear maybe..............


it is an Acid-fast bacillus smear showing characteristic cording in

Mycobacterium tuberculosis.



:to:

[UQU girl]

<center>

Thanx Rosa & SweetOne for ur pic & ur informaion

really we appreciate ur job.. & ur Knowledge.. thanks so much

& i wanna share u with what i have to

What is this cells? in which disease we can find it??

[RoSa]

<center>

oh my god .. really it's not clear and i can't gusse it :(

Dr.Asser, Mss.Abeer & Mr.Hamdi don't saw my falling please :(

maybe next time i will be better in gussing (than this) :D

Thank you again sweetone ..

and waiting for other pictures :)


Regards..

[RoSa]

<center>
UQU girl you are must welcome here :)

BuT..

I want to ask...

Is that smear made from bone marrow aspiration?

OR...

Is it a blood smear??

Regards..

[RoSa]

<center>
Where are you dear UQU girl ??

we still waiting :)

Regards..

[H@SSOOM]

Helloooooooooooooooooooooooooooo

I have moved this thread from Innovation center to Medical sciences section as its writer want

however


it still has many innovations

[RoSa]

<center>
thank you dr.hassom ..

و ياليت يتنشط الموضوع ..

نبض القلوب ..

تظرا لانه عندك ملف صور علمي كبير فأنا اوكل لك هذه المهمه .. و عارفه انك قدها ..

==> توريطه:)

يالله سلام

[RoSa]

<center>
Our colleges

Welcome back again

Operate your mind... Do not be shy and start with me

Search on your memory

Its there

So..

Again..

Guess with Us..

What is This???

This a gram stain slide..


If you failing to know it from this picture I will attach its plate...

So...

Be with us...

I'm waiting for you all

[RoSa]

<center>
all my thanks to dear Heart.. because she is the reason to activate this thread

ملحوظه: اللي يشوفلي غلط في اللغه ياليت ما يتردد ينبهني .. لأنه لو استمرينا في الغلط عمرنا ماراح نعدله :)

[Heart Pulse]

alsalam 3alykom

oooooh dear rosa

i need this thread

sleeping and plying will be affecting on my memory

and by the time you will see ABYAD YA WARD HeartPuls brain


:o:

ummmmmmmmm

lets me remember this slid

u said gram stain

so its bacterial

ummmm
ok heartpuls
which type of bacteria take curve shap???





i can not identify the color of slid
((too bad stain))
:sad:

so i ask u one q
the color is blue or red???


do not worry darling
i will be open my micro books and search

:eye:

with all my love rosa

HP

[RoSa]

<center>
Ok dear...

I will give you some information about it...

It cause an endemic disease..

It is a gram negative, highly motile, usually curved with a single flagellum at one end..

The best selective media for that organism is TCBS (give me the full name of this media)...

If you give me rights answer... I will attach other nice pictures for it :)

Keep on trying (thumb up)..

Good Girl...

Where is the others??

Join us and enjoy your time here :)

[miss_biology]

hi rosa



It is

vibrio cholerae


T.C.B.S

Thiosulphate Citrate Bile Salt sucrose


it is dark green in colour


right ?????

[RoSa]

<center>

You are right miss_biology

I will back

Be with us

[dodo]

Let me share U if U allow me...

What is this ???:?:

[dodo]

tek tek tek


is anybody there?


What happened >>???


come on !!
:O:

Guess what ?

[RoSa]

dodo

sorry for being late

this figure is illustrate trophozoite (a sporozoan in the active feeding stage of parasite life cycle) of Gardia Lamblia

one of the most famous trophozoite seen under microscope in saudi population

it is appear with the water contamination and it can be transmitted by faecal-oral route

***

whats next ???

[Love 4 Life]

hi

don't see the photo address

:85:


http://www.srs.dl.ac.uk/Annual_Reports/AnRep01_02/anthrax-bacteria.jpg


just try

hint:
it is GPB

[RoSa]

Excuse me

what GPB??

[Dr.zero]

Hi..every 1..
thanx Rosa 4 this great subject..
i love it :LL:

.....


and about the previous pic
i think he or she meant Gram Positive Bacilli (GPB)
....

and the pic i think for Bacillus Anthracis...that bacteria cause ANTHRAX..

am soooory ... maybe i little bit cheated :d:

cuz u know..am on my annual vaccation and my scientific mind out of order...loooooooooooool

.............


seee u all soooon


........

Salam

[Love 4 Life]

dr. zero


u r right


thanx

[Heart Pulse]

Alsalm Alykom

Heart Pulse is come back


:J:
I Know i did "NOTHING" for this section

But really the internship year is not an easy year

:LL:

any why,

I like this thread, coz it's always refresh my mind
and for my position , i need this kind of refreshment!!!!


OK

today I Have easy and practical Q

Q. What is the total magnification produced when using a 10X ocular lens and a 40X objective lens on a ligt microscopy?

a. 4,000X
b. 800X
c. 400X
d. Cannot be determined without additional information.

ummmmm i think the 1st and 2nd year student can answer my Q

yalla, waiting every body

see ya!!!

HP

[master_2007]

Thank You For Guess
I See Laboratory , Large Microscope And Girl And Some MEDICAL DEVICES

[الضنا]

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